手持式葉綠素熒光儀---FluorPen FP110用于實驗室、溫室和野外快速測量植物葉綠素熒光參數，具有便攜性強、精度高、性價比高等特點；雙鍵操作，具圖形顯示屏，內置鋰電和數據存儲，廣泛應用于研究植物的光合作用、脅迫監測、除草劑檢測或突變體篩選，還可用于生物毒理的生物檢測，如通過不同植物對土壤或水質污染的葉綠素熒光響應，找出敏感植物作為生物傳感器用于生物檢測。FP110配備多種葉夾型號，用于不同的樣品與研究。

應用領域

       手持式葉綠素熒光儀---FluorPen FP110適用于光合作用研究和教學，植物及分子生物學研究，農業、林業，生物技術領域等。研究內容涉及光合活性、脅迫響應、農藥藥效測試、突變篩選等。

• 植物光合特性研究
• 光合突變體篩選與表型研究
• 生物和非生物脅迫的檢測
• 植物抗脅迫能力或者易感性研究
• 農業和林業育種、病害檢測、長勢與產量評估
• 除草劑檢測
• 教學

功能特點

• 結構緊湊、便攜性強，LED光源、檢測器、控制單元集成于僅手機大小的儀器內，重量僅188g
• 功能強大，是葉綠素熒光技術的高新結晶產品，具備了大型熒光儀的所有功能，可以測量所有葉綠素熒光參數
• 內置了所有通用葉綠素熒光分析實驗程序，包括3套熒光淬滅分析程序、3套光響應曲線程序、OJIP快速熒光動力學曲線等
• 高時間分辨率，可達10萬次每秒，自動繪出OJIP曲線并給出26個OJIP–test參數
• FluorPen專業軟件功能強大，可下載、展示葉綠素熒光參數圖表，也可以通過軟件直接控制儀器進行測量
• 具備無人值守自動監測功能
• 內置藍牙與USB雙通訊模塊，GPS模塊，輸出帶時間戳和地理位置的葉綠素熒光參數圖表
• 配備多種葉夾型號：固定葉夾式（適于實驗室內暗適應或夜間快速測量）、分離葉夾式（適用于野外暗適應測量）、探頭式（透明光纖探頭，具備葉片固定裝置，用于非接觸性測量監測或光適應條件下的葉綠素熒光監測）、用戶定制式等
• 可選配野外自動監測式熒光儀，防水防塵設計

測量程序與功能

• Ft：瞬時葉綠素熒光,暗適應完成后Ft＝F₀
• QY：量子產額，表示光系統II 的效率，等于Fv/Fm(暗適應狀態)或ΦPSII (光適應狀態)。
• OJIP：快速熒光動力學曲線，用于研究植物暗適應后的快速熒光動態變化
• NPQ：熒光淬滅動力學曲線，用于研究植物從暗適應到光適應狀態的熒光淬滅變化過程。
• LC：光響應曲線，用于研究植物對不同光強的熒光淬滅反應。
• PAR：光合有效輻射，測量環境中植物生長可以利用的400-700nm實際光強（限PAR型號）。

應用案例

2017年4月，美國國家航空*（NASA）新一代*植物培養器（Advanced Plant Habitat，APH）搭載聯盟號MS-04貨運飛船抵達空間站。宇航員使用FluorPen手持儀葉綠素熒光儀在其中開展植物生理學及太空食物種植（growth of fresh food in space）的研究。

參考文獻

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附：OJIP參數及計算公式

Bckg = background

Fo: = F50µs; fluorescence intensity at 50 µs

Fj: = fluorescence intensity at j-step (at 2 ms)

Fi: = fluorescence intensity at i-step (at 60 ms)

Fm: = maximal fluorescence intensity

Fv: = Fm - Fo (maximal variable fluorescence)

Vj = (Fj - Fo) / (Fm - Fo)

Fm / Fo = Fm / Fo

Fv / Fo = Fv / Fo

Fv / Fm = Fv / Fm

Mo = TRo / RC - ETo / RC

Area = area between fluorescence curve and Fm

Sm = area / Fm - Fo (multiple turn-over)

Ss = the smallest Sm turn-over (single turn-over)

N = Sm . Mo . (I / Vj) turn-over number QA

Phi_Po = (I - Fo) / Fm (or Fv / Fm)

Phi_o = I - Vj

Phi_Eo = (I - Fo / Fm) . Phi_o

Phi_Do = 1 - Phi_Po - (Fo / Fm)

Phi_Pav = Phi_Po - (Sm / tFM); tFM = time to reach Fm (in ms)

ABS / RC = Mo . (I / Vj) . (I / Phi_Po)

TRo / RC = Mo . (I / Vj)

ETo / RC = Mo . (I / Vj) . Phi_o)

DIo / RC = (ABS / RC) - (TRo / RC)